To Kill a Mockingbird- Chapter 12

Mister Jem

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Calpurnia calling Jem Mister implies that Jem is growing up, he is no longer a child and therefore is changing his morals and behaviour. Jem is treating Scout differently, instead of telling Scout to stop being a girl, he is telling her to behave more like a lady. This symbolises that he is trying to fit into the stereotyping against men and women.